Reduction in Mcl-1 transcript and protein levels in CLL cells after treatment with forodesine and dGuo. CLL cells were incubated with or without 2μM forodesine and 20μM dGuo in suspension or on MSCs. The levels of Mcl-1 transcript (n = 8, A), and Mcl-1 protein (n = 19, B), were measured by real time RT-PCR and immunoblotting analysis, respectively. The Western blot images from 5 representative patients for Mcl-1 and Bcl-2 proteins (C). Actin was used as loading control. C indicates CLL; F, forodesine plus dGuo; M, M210B4; and N, NKtert.