Mobilization with clo-lip is independent of neutrophil proteases. (A) Concentration of proteolytically active NE and catG in BM fluids from clo-lip–treated C57BL/6 mice. Results are compared with 129Sv mice treated for 4 days with G-CSF (4 mice per time point; mean ± SD). (B) Gelatin zymography of BM fluids from C57BL/6 mice treated with clo-lip or G-CSF. Each lane contains a pool of BM fluids from 4 different mice. (C) Effect of G-CSF on osteoid surface per bone surface (OS/BS), osteoblast surface per bone surface (ObS/BS), and number of osteoblasts per bone perimeter (NOb/BPm) in DPP1-deficient mice. Data are means ± SEM of both tibias from 4 mice per group. Effect of clo-lip in GCSFR-deficient mice on mobilization of CFC in blood (D) and (E) concentrations of active NE, catG, and MMP-9 in BM fluids (4 mice per time point; mean ± SD). Effect of 4-day clo-lip treatment on osteoid surface per bone surface (OS/BS), osteoblast surface per bone surface (ObS/BS), number of osteoblasts per bone perimeter (NOb/BPm) (F), osteocalcin⁺ mature osteoblasts (G, right panels, metaphyseal trabecular bone), and F4/80⁺ osteomacs (G, left panels) in GCSFR knockout mice (means ± SEM and representative ×40 magnification images, respectively, 3 mice per group). *P < .05, **P < .01, and ***P < .001.