Constitutive or inducible B7-H1 expression in MDS cell lines.(A) B7-H1 mRNA expression analyzed by reverse-transcription polymerase chain reaction. Equal amounts of cDNA from 3 myelodysplastic syndromes (MDS) cell lines were amplified. Vertical lines have been inserted to indicate a repositioned gel lane. (B) B7-H1 expression analyzed by flow cytometry (FCM). The cell lines were cultured with medium alone or with IFNγ. Thick line histogram represents staining with anti–B7-H1 monoclonal antibodies; dotted line histogram represents staining with isotype-matched control Ig. (C) Effects of cytokines on B7-H1 expression in F-36P and SKM-1 cells. Data (relative fluorescence intensity [MFI]) are mean and SD of 3 experiments. Results without SD error bar indicate small SD.