Id2 knockdown in Gfi-1^−/− BMC partially rescues hematopoiesis. (A) pRetro-Id2-shRNA– or pRetro-NS-shRNA–transduced Gfi-1^+/+ or Gfi-1^−/− BMCs (1 × 10⁶ per recipient) with supporting wild-type BMC (2 × 10⁵ per recipient) were transplanted into lethally irradiated C57BL/6 mice. Myeloid reconstitution in recipient bone marrow was examined 4 weeks after transplantation by analyzing the GFP⁺ donor cells with Gr-1 and Mac-1 surface markers by flow cytometry. (B) Total cell numbers of Gr-1^dullMac-1⁺ and Gr-1^brightMac-1⁺ cells were shown. *P < .05 in 2-sided t test. Four recipient mice were used in each group. Three independent experiments were performed. (C) B-cell reconstitution in recipient bone marrow was examined 4 weeks after transplantation by analyzing the GFP⁺ donor cells with B220 and CD43 surface markers by flow cytometry. (D-E) Total cell number of B220⁺CD43⁺ and B220⁺CD43⁻ cells, and fold of induction of B220⁺ cells by Id2 shRNA were shown. *P < .05 in 2-sided t test. Four recipient mice were used in each group. Three independent experiments were performed.