Relative expression of cyclin D1 and Mll mRNA. The level of Mll and cyclin D1 mRNA expression was determined by real-time PCR after normalization for GAPDH. Graphs display representative experiment. (A) Relative levels of cyclin D1 mRNA in wt and Hhex^−/− CD41⁺CD45⁻c-kit⁺ cells. Cyclin D1 is down-regulated 1.3-, 2.7-, and 3.9-fold in CD41⁺CD45⁻c-kit⁺ cells at day 6 through 8, respectively. (A) Relative levels of Mll mRNA in wt and Hhex^−/− CD41⁺CD45⁻c-kit⁺ cells. Mll was down-regulated 1.1 at day 6, and 1.6 at days 7 and 8 of coculture.