Overexpressed miR-146a does not alter platelet activation. (A) Platelet-rich plasma was prepared from miR-146a–overexpressing and control mice. Binding of GFP⁺ and GFP^– platelets to Alexa 647-fibrinogen was measured by flow cytometry before and after stimulation with low and high doses of agonists ADP, convulxin (CVX), and PAR4 agonist peptide (AYP). No stim indicates unstimulated platelets. (B) Expression of chemokine receptor CXCR4 on control and miR-146a–overexpressing platelets, as measured by flow cytometry.