Flip deletion results in increased neutrophils and decreased macrophages in the peritoneum. Resident (A-B) or thioglycollate elicited (E-F) cells from peritoneal cavities were analyzed by flow cytometry, gaiting on CD11b⁺ cells. The macrophages (MΦ) were identified as F4/80⁺, Gr1⁻, whereas granulocytes (Gran) were identified as F4/80⁻, Gr1⁺. (B) The total number of cells, resident macrophages and granulocytes from controls (n = 20) and Flip^f/d, LysM^c/+ mice (n = 26) are summarized. (C) There was an inverse relationship between the percentage of peritoneal macrophages and granulocytes in the wild-type and Flip^f/d, LysM^c/+ mice. (D) PCR genotyping of granulocytes and macrophages isolated from peritoneal cavities were performed using the 3 PCR primers indicated in Figure 1. (F) The total number of thioglycollate elicited macrophages and granulocytes at 72 hours (n = 7 for each group) are summarized. *P < .05, **P < .01, and ***P < .001 between groups.