No evidence for residual HIV target cells of host origin in the liver and the brain. CCR5-expressing CD4⁺ T cells or macrophages were detected (A) in liver and (B) in brain tissue sections obtained 12 and 17 months after CCR5Δ32/Δ32 SCT, respectively, by in situ immunofluorescence double staining for CD4 (green) or CD68 (green) and CCR5 (red). Original magnification ×400. Images were acquired by use of the AxioImager Z1 fluorescence microscope (Carl Zeiss MicroImaging) coupled to the AxioCam MRm digital camera (Carl Zeiss). Acquisition software: Axiovision (Carl Zeiss). Software used for image processing: Adobe Photoshop CS (Adobe Systems).