Flow cytometry analyses of Th17 and of Tregs in the peripheral blood of 31 patients with acute, chronic, or no chronic GVHD. Flow cytometry analyses of Th17 and of Tregs in the peripheral blood of 23 patients with acute, chronic, or no chronic GVHD. PBMCs from healthy controls (n = 5) and patients with GVHD (acute, n = 12 and chronic, n = 3) or without (n = 8) were stained with anti-CD3, anti-CD4, anti-CD25 antibodies followed by intracellular Foxp3 antibody and examined by flow cytometry. Intracellular IL-17 antibody was also examined by flow cytometry after stimulation for 6 hours with phorbol myristate acetate and ionomycin. (A) Frequencies of Tregs in patients and controls. (B) Frequencies of Th17 T cells in patients and controls. (C) Th17/Tregs ratio in patients and controls. The mean value of each group is represented (solid line).