A noncoding region in the CCR6 gene displays differential methylation in human PBMCs. (A) Localization of the CpG-rich region within the human CCR6 locus. The exon structure of the putative CCR6 transcripts, obtained from the Ensembl database (GRCh37), were plotted together with a homology chart (human-mouse; Vista), predicted promoter regions (Genomatix) and the identified DMR in the CCR6 locus. The gray boxes in the CCR6 transcripts represent noncoding exon regions, whereas the black boxes contain coding regions. (B) DNA methylation pattern for CCR6 were analyzed in sorted CD4⁺CD45RA⁺ and CD4⁺CD25^high T cells from pooled PBMCs (5 donors). Each column represents one blood cell subset with each row representing a single CpG site. The numbers indicate the position of the CpG motif as indicated in supplemental Figure 2. DNA methylation was measured by bisulfite sequencing. CpG methylation levels are color-coded according to a scale ranging from yellow (0% methylation) to blue (100% methylation).