Demethylation of the CCR6 region correlates with CCR6 expression in human CD4⁺ and CD8⁺ T cells. (A) Representative FACS staining of CCR6 and CD45RA on CD4⁺CD25⁻ and CD8⁺CD25⁻ lymphocytes. (B) CD4⁺ and CD8⁺ cells were isolated from PBMCs and sorted into CCR6⁻CD45RA⁺ naive and CCR6⁻ and CCR6⁺CD45RA⁻ memory T cells resulting in subsets with a purity more than 95%. Methylation analyses of sorted subsets are shown for one representative donor. The degree of methylation is color-coded according to the scale in Figure 1B. Graphs below show the mean of methylation from all CpG sites from 5 to 6 donors for CD4⁺ T cells and 3 donors for CD8⁺ T cells, respectively.