Characterization of chromosomal aberrations affecting the TERT-CLPTM1L locus. IGH FISH (A) and R-banding analysis (B) of the same metaphase of case 1 showing the cytogenetic cryptic t(5;14). (C) Interphase FISH showing a fusion of IGH and the TERT-CLPTM1L locus in case 1. (D-F) Schematic maps of the breakpoint regions in cases with t(5;14)(p15;q32) based on FISH mapping. Black bars indicate the hybridized BAC and Fosmid clones; gray bars represent the genes in the region. The breakpoint regions were determined by the respective FISH hybridization patterns. (G) FISH using the TERT-CLPTM1L 3-color break-apart assay on MCL case 10 showing break in the clone RP11-117B23 (blue signal). The R-banding image with Chromomycin A3 (C2659, Sigma-Aldrich) and Methyl green (M8884, Sigma-Aldrich) was obtained using a 63×/1.40 numeric aperture oil objective in a Zeiss Axioskop Imager M1 fluorescence microscope (Zeiss) with R-banding filter sets (Zeiss) and documented using the IKAROS imaging system version 5.2.11 (MetaSystems). FISH images were acquired using a 63×/1.40 numeric aperture oil objective in a Zeiss Axioskop 2 fluorescence microscope (Zeiss) equipped with the appropriate filter sets (AHF) and documented using a CV-M300 camera (JAI Corporation) and the ISIS imaging system Version 5.2.11 (MetaSystems). dist indicates distal; and prox, proximal.