Inhibitory effects of PPAR-γ activation are mediated via the Card9/NF-κB signaling pathway. Nuclear extracts and whole-cell lysates were prepared from mDCs treated with TGZ or DMSO and stimulated with curdlan. The nuclear translocation of NF-κB member RelB was inhibited after PPAR-γ activation (A). The expression of Card9 was reduced after TGZ treatment and was correlated with the RelB translocation (B). The transcription factor NFAT was not affected by PPAR-γ activation (C). The data shown are representative of at least 3 independent experiments.