Figure 4
miR-21 rescued osteoclastogenesis in DGCR8-deficient BMMs. (A) Immunoblotting analysis of DGCR8 of DGCR8F/F BMMs (pMX-IB) and DGCR8-deficient BMMs (pMX-Cre-IB) without RANKL treatment. (B) QRT-PCR was performed with primer for miR-21. The PCR products were normalized to U6 level for each reaction. The data represent the means ± SDs of 3 experiments in triplicate. WT indicates DGCR8 wt BMMs. (C) Immunoblotting analysis of PDCD4 in infected BMMs without RANKL treatment. (D) TRAP-positive multinucleated cells counted at 3 days after RANKL treatment, cultured in 24-well plates (200×). Similar findings were obtained in 4 independent sets of experiments. (E) RT-PCR analysis of the genes related to osteoclastogenesis. OC indicates osteoclast.
