TF is associated with cell-derived vesicles in saliva. Microparticles and exosomes were isolated from cell-free saliva (n = 10). (A) Western blot showing the presence of TF in fractions of microparticles and exosomes, but below the detection limit in the corresponding supernatant saliva. Data from 3 persons are shown. (B) Representative fluorescence histograms of microparticles (left), exosomes (middle), and supernatant (right) stained with control antibody (filled) or anti-TF (open). (C) TEMs of isolated microparticles (> 100 nm diameter, top left) and exosomes (< 100 nm, top right) stained with anti-TF followed by immunogold labeling. Representative controls of vesicles labeled with control antibody (IgG) plus immunogold staining (bottom left), or immunogold staining alone (goat anti–mouse [GAM], bottom right).