Panel of 11 genes and the molecular risk algorithm. (A) The molecular risk algorithm is determined on the basis of the relative contributions of each of the 4 gene functional groups from the tumoral HRS and their reactive microenvironment as follows: MRS = exp (fx)/(1 + exp [fx]), where fx = (−0.913) + (0.401 × apoptosis) + (0.284 × cell cycle) + (−0.301 × monocyte) + (−0.143 × IRF4). Coefficients were derived from a multivariate analysis in which positive values indicate that a greater level of expression is correlated with a worse outcome, and negative coefficients indicate that a greater level of expression of the pathways is associated with a better outcome. (B) MRS as a continuous function was used to set a threshold for stratifying patients by ROC analysis. Patients were stratified according to the levels of the molecular risk score into low-risk (< 0.3) and high-risk (≥ 0.3) groups. (C-D) Survival estimates of FFS in patients from estimation (n = 183) and validation (n = 79) sets after classification into risk groups. Kaplan-Meier analysis and the log-rank test gave significant results in both estimation and validation sets, indicating the potential prognostic capacity of the algorithm developed here.