Effect of washing of aged platelets before transfusion on cytokine and chemokine concentrations in the lungs. Concentrations of IL-6 and CINC-3 in the lung homogenate of animals receiving saline or LPS as first hit and subsequently infusion of saline (control), supernatant (sup) of aged PLT products, or washed (wash) aged platelets. Data are presented as mean ± SEM. No differences are seen between supernatant of aged PLT products and washed aged platelets on lung inflammation in both saline and LPS-pretreated animals.