Tunicamycin-induced alterations in the proliferation and differentiation of G193X Elane granulocytic precursors. Cultured granulocytic precursors were treated with tunicamycin (0.5 μg/mL) or vehicle alone on day 0. (A) Real-time RT-PCR for Hsap5, Chop, and Herpud was performed on cells 24 hours after exposure to tunicamycin (TN) or vehicle alone (Ctrl). RNA expression relative to β-actin is shown. (B) Viable cells were quantified by flow cytometry as annexin V⁻ 7-actinomycin D⁻ cells. (C) Manual leukocyte differentials (200-cell count) were performed on day 4 after treatment with tunicamycin. Neutrophil indicates segmented and band neutrophils; Pro/Mye, promyelocytes and myelocytes. Data represent the means ± SEM of 4-8 samples. *P < .05 compared with saline-treated cells of the same genotype; ^#P < .05 compared with wild-type mice in the same treatment group.