RBL2 silencing contributes to cell cycle deregulation in vitro and in vivo. (A) Soft agar assay: Comparison between the ability of empty vector–transfected cells and pcDNA3-MYC, pshRb2, or double-transfected cells to growth in soft agar. Error bars represent standard deviation between triplicates. In double transfectants, we observed significantly higher colony numbers than in single ones (P = .04). (B) Matrigel invasion assay: Comparison between the ability of empty vector–transfected cells and pcDNA3-MYC, pshRb2, or double-transfected cells to cross the extracellular matrix. Error bars represent standard deviation between triplicates. In double transfectants, significantly higher migration was detected (P = .05). (C) In vivo test indicated higher engraftment capability for double transfectants than for single ones.