hESC- and fetal liver–derived CD14⁺CD16⁺ cells demonstrate a nonclassic monocyte phenotype. Immunophenotype analysis of hESC-derived (top left panel), CD34⁺ fetal liver–derived (top right panel), and CD34⁺ adult-derived (bottom panel) CD14⁺CD16⁺ cells. The percentages of cells expressing the indicated markers within CD14⁺ cells are shown. The majority of embryonic and fetal subsets expressed CCR5 and CX3CR1. When monocytic cells were generated from adult CD34⁺ cells, a marker of the inflammatory type, CCR2, was detected on a subset of cells. Gating was set according to the negative isotype controls. One of at least 3 representative experiments is shown. The corresponding cell frequencies as determined in 3 independent experiments are presented.