Iron indices in wild-type (WT), Hfe^−/−, Tfr2^−/−, and Hfe^−/−/Tfr2^−/− mice treated with LPS. (A) Serum iron, (B) transferrin saturation, (C) HIC, and (D) hepatic ferritin levels were measured in 5-week-old male WT, Hfe^−/−, Tfr2^−/−, and Hfe^−/−/Tfr2^−/− mice injected with saline (white bars) or LPS (black bars) for 6 hours (n = 3-4 per group). Data are shown as the mean; error bars indicate SEM. Statistical comparisons were performed using the Student t test. Significant differences (P < .05) are denoted between control and LPS treatments (*) and between strains compared with WT (a), with Hfe^−/− (b), and with Tfr2^−/− (c).