Splenic ferroportin expression and splenic iron concentration in WT, Hfe^−/−, Tfr2^−/−, and Hfe^−/−/Tfr2^−/− mice treated with LPS. (A) Western blotting was used to determine the expression of ferroportin in the spleens of 5-week-old male WT, Hfe^−/−, Tfr2^−/−, and Hfe^−/−/Tfr2^−/− mice injected with saline or LPS for 6 hours. The blots in panel A were quantitated and results are shown relative to Gapdh for saline-injected (white bars) and LPS-injected (black bars) mice (B). Splenic iron concentration was determined in all mice (C). Data are shown as the means; error bars indicate SEM. Statistical comparisons were performed using the Student t test. Significant differences (P < .05) are denoted between control and LPS treatments (*) and between strains compared with WT (a), with Hfe^−/− (b), and with Tfr2^−/− (c).