Figure 6
Figure 6. Comparison of TFPIWT, TFPI-ΔK3, and TFPIK3P1 in FXa-induced coagulation of plasma. Assays were performed as described.27 Briefly, a 50/50 mixture of TFPI and plasma was incubated at 37°C for 3 minutes before adding a second mixture containing FXa, CaCl2, and cephalin, also preincubated at 37°C for 3 minutes, and the clotting time recorded using a fibrometer. Final concentrations for each reagent were TFPI (0-20nM), FXa (2nM), cephalin (25μM), and CaCl2 (5mM). Assays were performed in duplicate. (A) Assay with PS-deficient plasma (B) Assay with pooled normal plasma. (C) Assay with PS-deficient plasma spiked with 200nM PS. ● indicates TFPIWT; □, TFPIK3P1; and ▵, TFPI-ΔK3.

Comparison of TFPIWT, TFPI-ΔK3, and TFPIK3P1 in FXa-induced coagulation of plasma. Assays were performed as described.27  Briefly, a 50/50 mixture of TFPI and plasma was incubated at 37°C for 3 minutes before adding a second mixture containing FXa, CaCl2, and cephalin, also preincubated at 37°C for 3 minutes, and the clotting time recorded using a fibrometer. Final concentrations for each reagent were TFPI (0-20nM), FXa (2nM), cephalin (25μM), and CaCl2 (5mM). Assays were performed in duplicate. (A) Assay with PS-deficient plasma (B) Assay with pooled normal plasma. (C) Assay with PS-deficient plasma spiked with 200nM PS. ● indicates TFPIWT; □, TFPIK3P1; and ▵, TFPI-ΔK3.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal