Figure 4
Figure 4. Effect of C1 domain mutations on parameters of the factor Xase complex. (A) Vmax was obtained with saturating factor IXa and factor X. (B) Apparent factor IXa affinities were measured by various factor IXa concentrations in the presence of factor VIII (0.15 U/mL) and excess phospholipid containing 4% or 15% PS. (C) Apparent factor X affinities were determined by various factor X concentrations in the presence of excess phospholipid vesicles and factor IXa. The apparent KD values were determined using nonlinear least squares analysis with the software GraphPad Prism, Version 5.0. Data are mean ± SEM for 2 to 4 experiments, each performed in duplicate.

Effect of C1 domain mutations on parameters of the factor Xase complex. (A) Vmax was obtained with saturating factor IXa and factor X. (B) Apparent factor IXa affinities were measured by various factor IXa concentrations in the presence of factor VIII (0.15 U/mL) and excess phospholipid containing 4% or 15% PS. (C) Apparent factor X affinities were determined by various factor X concentrations in the presence of excess phospholipid vesicles and factor IXa. The apparent KD values were determined using nonlinear least squares analysis with the software GraphPad Prism, Version 5.0. Data are mean ± SEM for 2 to 4 experiments, each performed in duplicate.

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