Figure 5
Figure 5. miRNAs 381 and 539 are repressed with KIT signaling in mast cells. (A) Schematic representation of the murine genomic region of chromosome 12 with the conserved miRNAs for 381, 487b, 539, and 544. (B) Schematic representation of the murine MITF 3′-UTR depicting phylogenetically conserved binding sites for miR-381 and miR-539. (C) Real-time TaqMan PCR showing the depression of mature miR-381 and miR-539 with activation of KIT in primary BMMCs treated with SCF. *P < .05. (D) Real-time TaqMan PCR showing the increase in mature miR-381 and miR-539 levels with inhibition of KIT in murine mastocytoma (P815) with imatinib. **P < .005. Signals were normalized to miR-16 expression. P values are calculated with Student t test; all experiments performed in triplicate.

miRNAs 381 and 539 are repressed with KIT signaling in mast cells. (A) Schematic representation of the murine genomic region of chromosome 12 with the conserved miRNAs for 381, 487b, 539, and 544. (B) Schematic representation of the murine MITF 3′-UTR depicting phylogenetically conserved binding sites for miR-381 and miR-539. (C) Real-time TaqMan PCR showing the depression of mature miR-381 and miR-539 with activation of KIT in primary BMMCs treated with SCF. *P < .05. (D) Real-time TaqMan PCR showing the increase in mature miR-381 and miR-539 levels with inhibition of KIT in murine mastocytoma (P815) with imatinib. **P < .005. Signals were normalized to miR-16 expression. P values are calculated with Student t test; all experiments performed in triplicate.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal