E47-deficient HSCs display poor self-renewal efficiency in vivo. Serial transplantation was performed to examine the long-term self-renewal efficiency of HSCs from WT and E47 KO mice. The bone marrow cells from WT and E47 KO littermates were first adoptively transferred into primary recipients for 16 weeks and then serially transferred into secondary recipients. Blood reconstitution was measured monthly. Recipients were killed at 16 weeks after transplantation, and lineage reconstitution in the spleen was examined. Myeloid or lymphoid reconstitution efficiency was indicated by the number of donor-derived CD45.2⁺ Gr-1⁺ cells (A) or CD3⁺ cells (B), respectively (n = 6 to 8 mice per group). **P < .05. ns indicates not significant.