Figure 4
Figure 4. LAP and LAHP thymic lymphomas exhibit distinct patterns of genomic instability. (A) LAP thymic lymphomas harbor clonal translocations. Shown are spectral and computer-classified images of the clonal translocations observed in 5 of the LAP tumors (nos. 144, 91, 34, 51, and 734) and the short arm fusions found in 2 of the LAP tumors (nos. 144 and 34) by SKY. Also shown is the karyotype table of one metaphase from LAP tumor 688 demonstrating aneuploidy but no apparent translocations. The identities of the chromosomes participating in these translocations or fusions are indicated. DAPI images are depicted for the chromosome fusions within LAP tumors 144 and 34 to reveal the structure of these chromosome lesions. DAPI images also are shown for the normal chromosome 9 and t(9;16) translocation in LAP tumor 734 to reveal a centromere-like structure on the chromosome 9 portion of the t(9;16) translocation. (B) LAP thymic lymphomas contain clonal Tcrα/δ translocations. Shown are representative FISH and chromosome paint analyses of LAP tumors with clonal chromosome 14 translocations (nos. 144, 51, and 34) to evaluate for potential involvement of Tcrα/δ loci (tumors 144, 51, and 34) and Tcrβ loci (tumor 34). All images were counterstained with DAPI to visualize chromosomes. Arrows indicate translocation sites that involve Tcrα/δ or Tcrβ loci. For spectral karyotyping (SKY), whole chromosome painting (WCP), and FISH analysis, metaphases were prepared using standard protocols (Coldemid treatment), and then hybridized with SKY and WCP probes from Applied Spectral Imaging according to manufacturers' instructions, and/or with labeled BAC FISH probes according to standard protocols. (C) LAHP thymic lymphomas harbor clonal translocations. Shown are spectral and classified images of the clonal translocations observed in each of the 7 LHP tumors (nos. 215, 887, 702, 519, 104, 300, and 521) analyzed by SKY. The identities of the chromosomes participating in these translocations are indicated. For tumor 215, a normal chromosome 6 and a normal chromosome 12 are shown to reveal the chromosome 6 fragment and the elongated chromosome 12.

LAP and LAHP thymic lymphomas exhibit distinct patterns of genomic instability. (A) LAP thymic lymphomas harbor clonal translocations. Shown are spectral and computer-classified images of the clonal translocations observed in 5 of the LAP tumors (nos. 144, 91, 34, 51, and 734) and the short arm fusions found in 2 of the LAP tumors (nos. 144 and 34) by SKY. Also shown is the karyotype table of one metaphase from LAP tumor 688 demonstrating aneuploidy but no apparent translocations. The identities of the chromosomes participating in these translocations or fusions are indicated. DAPI images are depicted for the chromosome fusions within LAP tumors 144 and 34 to reveal the structure of these chromosome lesions. DAPI images also are shown for the normal chromosome 9 and t(9;16) translocation in LAP tumor 734 to reveal a centromere-like structure on the chromosome 9 portion of the t(9;16) translocation. (B) LAP thymic lymphomas contain clonal Tcrα/δ translocations. Shown are representative FISH and chromosome paint analyses of LAP tumors with clonal chromosome 14 translocations (nos. 144, 51, and 34) to evaluate for potential involvement of Tcrα/δ loci (tumors 144, 51, and 34) and Tcrβ loci (tumor 34). All images were counterstained with DAPI to visualize chromosomes. Arrows indicate translocation sites that involve Tcrα/δ or Tcrβ loci. For spectral karyotyping (SKY), whole chromosome painting (WCP), and FISH analysis, metaphases were prepared using standard protocols (Coldemid treatment), and then hybridized with SKY and WCP probes from Applied Spectral Imaging according to manufacturers' instructions, and/or with labeled BAC FISH probes according to standard protocols. (C) LAHP thymic lymphomas harbor clonal translocations. Shown are spectral and classified images of the clonal translocations observed in each of the 7 LHP tumors (nos. 215, 887, 702, 519, 104, 300, and 521) analyzed by SKY. The identities of the chromosomes participating in these translocations are indicated. For tumor 215, a normal chromosome 6 and a normal chromosome 12 are shown to reveal the chromosome 6 fragment and the elongated chromosome 12.

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