LAHP thymic lymphomas exhibit increased genomic instability compared with LAP and LHP tumors. (A) Spectral and DAPI images of a representative metaphase spread prepared from LAHP tumor 702. Circles represent chromosome fragments and/or detached centromeres. Slides were examined under a BX61 microscope (Olympus) and controlled by a LAMBDA 10-B Smart Shutter from Sutter Instrument. All images were captured using a 60×/1.42 oil objective lens, with a LAMBDA LS light source from Sutter Instrument, and a COOL-1300QS camera from Applied Spectral Imaging, and then analyzed and managed through Case Data Manager Version 5.5, installed and configured by Applied Spectral Imaging. Figure preparation was done using Photoshop and Illustrator from Adobe. (B-C) Quantitative analyses of nonclonal translocations (B) or chromosome fragments and detached centromeres (C) in LHP, LAP, and LAHP thymic lymphomas as analyzed by SKY. Graphs represent the average number of each type of chromosome abnormality observed per metaphase in tumors of each genotype. Dots represent the values for each tumor. Lines indicate the mean values for tumors of each genotype.