Effect of NK314 on growth of M059 cell lines. (A) Expression of DNA-PK complex in M059K, M059J (a DNA-PKcs mutant), MJ NA (mock vector-transfected), and MJ L24 (DNA-PK cDNA-transfected) cells was detected by Western blot analysis. M059 cells were treated with etoposide or NK314 in combination with or without NU7026 (NU). Effects of NK314 or etoposide on cell growth of M059K (B), M059J (C), MJ L24 (D), or MJ NA (E) were determined with Cell Counting Kit-8. Relative ratio of cell growth was calculated by dividing by the number of untreated cells and expressed as mean ± SD of triplicate analyses.