IP₃R dependence of Ca²⁺ oscillations induced by TAT-IDP and TAT-IDP_DD/AA. (A) Percent inhibition of peptide (10μM)–induced Ca²⁺ oscillations by the IP₃R inhibitor xestospongin C (10μM) or the phospholipase C inhibitor U73122 (0.25μM) in Jurkat cells, based on the percentage of cells displaying Ca²⁺ oscillations during 90 minutes of single-cell recordings (mean ± SE, 3 experiments, average 85 cells analyzed per treatment condition per experiment). (B) Representative single-cell Ca²⁺ recordings illustrating Ca²⁺ responses to 5μM peptide addition (arrow) in wild-type DT40 cells. (C) Percentage of wild-type (WT) and TKO DT40 cells displaying Ca²⁺ oscillations in response to treatment with 5μM peptides (mean ± SE, 4 experiments, average 60 cells analyzed per recording).