Identification of MMSET target genes by gene expression array. (A) Comparison of gene expression dataset from N-terminal knock down and MMSET II add back (t test paired; > 1.5-fold, P < .05). (B) Comparison of gene expression dataset from knockout cell line repleted with wild-type, active mutant, or dead mutant forms of MMSET (> 1.5-fold, P < .05). (C) Validation of MMSET target genes by real-time PCR. A representative experiment showing the regulation of JAM2 and GLS2 genes in the presence or absence of MMSET using the knockdown, knockout, and repletion systems. (D) Classification of MMSET target genes by Ontology analysis was performed using DAVID Ontology and Ingenuity Pathway Analysis. MMSET regulates genes mainly related with apoptosis, cell cycle, and DNA repair.