MMSET binding across genes overlaps with high levels of H3K36me2 and low levels of H3K27me3. Chromatin of MMSET shRNA-inducible cells grown with or without doxycycline for 7 days was immunoprecipitated with anti-MMSET, anti-H3K27me3, anti-H3K36me2, or anti-H3K36me3 antibodies. Immunoglobulin G was used as negative control (not shown). Eluted DNA was PCR amplified with primers designed across JAM2 (A) and GLS2 (B) loci.