Expression of Prdm16 in adult BM. (A) Schematic representation of the genomic Prdm16 region in Prdm16^{Gt(OST67423)Lex} (Prdm16^−/−) mice. Adapted from Zambrowicz et al¹⁹  with permission. (B) Expression of LacZ, measured by flow cytometry after staining with fluorescein di-D-β-galactopyranoside, in the BM of Prdm16^{Gt(OST67423)Lex/+} mice. CMP indicates common myeloid progenitor; GMP, granulocyte/macrophage progenitor; MEP, megakaryocyte erythroid progenitor; LT-HSC, long-term hematopoietic stem cell; and ST-HSC, short-term hematopoietic stem cell. (C) Expression of Prdm16 mRNA in HSCs (LSKFlt3⁻, composed of ST- and LT-HSCs), MPPs, CLPs, and lineage⁺ cells (n = 3). (D) Expression of Prdm16 mRNA in brain from Prdm16^−/−, Prdm16^+/−, and wt embryos.