American Society of Hematology

Figure 2

$Figure 2. Hematologic profile of Prdm16-deficient mice. (A) Cellularity of FL and thymus, and of adult BM, thymus, and spleen in Prdm16-deficient mice (n = 3–5). (B) WBC count (left panel) and lineage distribution (measured by flow cytometric analysis of CD19, Thy1, and Mac1/Gr1) in the PB of adult Prdm16+/− and wt mice (n = 5). *P = .0006. (C) Representative example of flow cytometric analysis of the fraction of LSK HSPCs, LSK CD150−Flt3+ MPPs, and LSK CD150+Flt3− HSCs in E15 Prdm16−/− and wt FL cells. (D) Frequency of the populations in panel C in E13.5 to E15.5 FL from Prdm16−/− and wt embryos. Frequencies obtained as the percentage of cells in doublet discriminated scatter (n = 4-6). *P < .05, one-way analysis of variance. (E) Frequencies of subpopulations of LSK cells in the BM of Prdm16+/− mice or wt littermates. Lineage cocktail included anti–CD41 and CD48 (n = 8). *P < .03.$

Hematologic profile of Prdm16-deficient mice. (A) Cellularity of FL and thymus, and of adult BM, thymus, and spleen in Prdm16-deficient mice (n = 3–5). (B) WBC count (left panel) and lineage distribution (measured by flow cytometric analysis of CD19, Thy1, and Mac1/Gr1) in the PB of adult Prdm16^+/− and wt mice (n = 5). *P = .0006. (C) Representative example of flow cytometric analysis of the fraction of LSK HSPCs, LSK CD150⁻Flt3⁺ MPPs, and LSK CD150⁺Flt3⁻ HSCs in E15 Prdm16^−/− and wt FL cells. (D) Frequency of the populations in panel C in E13.5 to E15.5 FL from Prdm16^−/− and wt embryos. Frequencies obtained as the percentage of cells in doublet discriminated scatter (n = 4-6). *P < .05, one-way analysis of variance. (E) Frequencies of subpopulations of LSK cells in the BM of Prdm16^+/− mice or wt littermates. Lineage cocktail included anti–CD41 and CD48 (n = 8). *P < .03.

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