FVIIa protects mice from increased vascular permeability in LPS challenge. (A) C57J BL (n = 11-13) mice were injected with saline (open bars) or LPS (5 mg/kg; black bars) intraperitoneally. In a third group, FVIIa (250 μg) was administered via tail vein to mice 2 hours before the LPS challenge (gray bars). (B) C57J BL mice (n = 3-5) were injected with saline, LPS, and FVIIa as described in panel A. In an additional group, FVIIa was not administered before, but instead was given 10 hours after LPS injection (hatched bars/last bar in each group). Vascular permeability in various tissues was measured 18 hours after LPS challenge by injecting 0.1 mL of 1% Evans Blue dye 45 minutes before the termination of the experiment and measuring the extravasation of dye into tissues. He indicates heart; Lu, lung; Ki, kidney; Sp, spleen; and Pl, plasma. *Statistically significant difference (P < .05) compared with mice challenged with LPS but not treated with FVIIa.