Figure 2
Figure 2. Hypoxia does not induce sVEGFR-1 production from neutrophils or HUVECs. (A) Human peripheral blood neutrophils were stimulated with 100 ng/mL GM-CSF at ambient O2 or 0.5% O2. Cell-free culture supernatants were collected at 48 hours and analyzed for sVEGFR-1 content by ELISA. (B) HUVECs were treated with 100 ng/mL GM-CSF at ambient O2 or 0.5% O2. sVEGFR-1 secretion after 48 hours was assessed by ELISA. *P < .001 versus all conditions shown.

Hypoxia does not induce sVEGFR-1 production from neutrophils or HUVECs. (A) Human peripheral blood neutrophils were stimulated with 100 ng/mL GM-CSF at ambient O2 or 0.5% O2. Cell-free culture supernatants were collected at 48 hours and analyzed for sVEGFR-1 content by ELISA. (B) HUVECs were treated with 100 ng/mL GM-CSF at ambient O2 or 0.5% O2. sVEGFR-1 secretion after 48 hours was assessed by ELISA. *P < .001 versus all conditions shown.

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