Characterization of Δ122p53 mice. (A) Schematic diagram comparing full-length p53 and Δ122p53. Domains are identified as follows: transactivation (TAD), proline-rich (PRD), DNA binding, tetramerization (4D), and C-terminal regulatory (CT). (B) Protein alignment between human Δ133p53 and mouse Δ122p53 illustrating the high homology between the 2 protein sequences. (C) To detect Δ122p53 protein, adipocytes from p53^+/+, p53^−/−, and Δ122p53 mice were left untreated (−) or treated (+) with 1 μg/mL amsacrine for 5 hours, and expression was determined by Western blotting using antibodies PAb240 and 1C12. β-Actin (antibody AC-15) was used as a loading control. Experiments were repeated to investigate at least 3 mice per genotype. (D) Δ122p53 shows nuclear and cytoplasmic locations. Δ122p53-expressing fibroblasts or p53 null fibroblasts were fixed and then stained with DAPI and p53 antibody PAb240 and with an AlexaFluor-488 conjugated second antibody. Images were taken with an Olympus BX51-TRF fluorescent microscope with 20×/0.5 objective lens. They were captured using a SPOT Camera (SP 6.0) and Software Version 4.0.1. Images were cropped using Photoshop Version CS2 (Adobe).