Localization of intestinal mast cells is abnormal in wild-type mice transplanted with N2-MxcKO-Ly5.1 bone marrow cells, reminiscent of that in N2-MxcKO mice. (A) Bone marrow cells from either N2-MxcKO- Ly5.1 mice or littermate Notch2^flox/flox- Ly5.1 mice were transplanted into lethally irradiated (9.5 Gy) C57BL/6-Ly5.2 mice. Toluidine blue staining, followed by eosin. Original magnification ×200. (B) The numbers of mast cells per 10 vcus distributing to various layers of the small intestine. Data are presented as means ± SEM; Mast cells in C57BL/6-Ly5.2 mice transplanted with Notch2^flox/flox-Ly5.1 (n = 3) versus N2-MxcKO-Ly5.1 (n = 3). P = .020594 (total) and P = .030123 (villus, lamina propria).