Chimerism and gene marking of donor-derived CD45.1⁺ peripheral blood leukocytes, splenocytes, and bone marrow cells. (A) eGFP-marking rates in STIF-cultivated Lin⁻ cells of pretransplantation samples. Because of limited cell numbers, data of G9-14 and G48-14 represent pooled samples 4-5 days after transduction. Individual data points in G9-168 and G48-168 represent eGFP marking in individually transduced and transplanted batches of cells at the day of transplantation. (B) Chimerism analysis of peripheral blood by staining donor-derived CD45.1 (2, 4, 6, and 9.5 months after transplantation). (C) Percentage of donor-derived (CD45.1⁺) eGFP-expressing cells in peripheral blood leukocytes over time. (D-E) Chimerism (D) and gene marking rate (E) in donor-derived splenocytes 9.5 months after transplantation. (F-G) Chimerism (F) and gene marking rate (G) in donor-derived bone marrow cells 9.5 months after transplantation.