Immunofluorescence microscopy of BHK-M cells expressing wt-fVIII and N1922S-fVIII. BHK-M cells, BHK-M cells expressing wt-fVIII, or BHK-M cells expressing N1922S-fVIII were incubated with anti-A2 MAb 2-54, ER probe polyclonal rabbit anti-BiP antibody (A) and the Golgi probe wheat germ agglutinin–Alexa Fluor 350 conjugate (B). The primary antibodies were detected with DyLight 488 donkey anti–mouse and DyLight 549 donkey anti–rabbit secondary antibodies. Nuclear staining was performed using DRAQ5. Images were captured with a Nikon Eclipse Ti inverted microscope using a 60× oil-immersion lens.