Tax trafficking between SUMO-enriched nuclear bodies. (A) HeLa cells were cotransfected with Tax and HA-ubiquitin and stained by immunofluoresence with anti–HTLV-I sera (blue), anti-HA polyclonal antibody (red) and anti–γ-tubulin mAb (green). Original colors of the immunofluoresence have been changed for clarity reasons. The 2 lanes represent different sections of the same cell. (B) HeLa cells were cotransfected with Tax-Dendra2 (2 μg) and SUMO1-YFP (50 ng). Irreversible photoconversion from green to red was performed on selected nuclear bodies 4 hours after transfection using a 405 laser. Cells were imaged using a LSM510 META laser confocal microscope in the green (lane 1) and red channels (lane 2) before and after photoconversion. Scale bar, 10 μm. (C) HeLa cells were transfected with Tax or empty vector and stained by double immunofluoresence with anti-Tax mAb (green) and anti-SUMO1 polyclonal antibody (red).