Figure 5
Figure 5. Unimpaired caspase activation of FU-Lcktg Lck−/−Lckind thymocytes in response to DNA damage. Thymocytes from 12-week-old Lck−/−/Lckind, Lcktg/Lck−/−/Lckind, and FU-Lcktg/Lck−/−/Lckind mice were subjected to 1000 Rad in a γ-irradiator. Irradiated and nonirradiated control cells were cultured for 4 hours in complete culture medium and cell lysates prepared. Western blot analysis was performed to assess activation of caspase 3 as judged by the appearance of the p17 active form and the reduction in the intensity of the p32 pro-form of the enzyme. Reprobes for ERK2 acted as a loading control. The data shown represent data from 1 of 3 FU-Lcktg/Lck−/−/Lckind mice.

Unimpaired caspase activation of FU-LcktgLck−/−Lckind thymocytes in response to DNA damage. Thymocytes from 12-week-old Lck−/−/Lckind, Lcktg/Lck−/−/Lckind, and FU-Lcktg/Lck−/−/Lckind mice were subjected to 1000 Rad in a γ-irradiator. Irradiated and nonirradiated control cells were cultured for 4 hours in complete culture medium and cell lysates prepared. Western blot analysis was performed to assess activation of caspase 3 as judged by the appearance of the p17 active form and the reduction in the intensity of the p32 pro-form of the enzyme. Reprobes for ERK2 acted as a loading control. The data shown represent data from 1 of 3 FU-Lcktg/Lck−/−/Lckind mice.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal