Defective expansion of CD45-deficient NK cells during MCMV infection. (A) WT and CD45-deficient mice were infected with 5 × 104 PFU of MCMV. Splenocytes gated on NK1.1+CD3− NK cells. Data are the average ± SD of results from 3-9 individual experiments. Percentages above each bar graph are the percentage increase of Ly49H+ NK cells in infected mice compared with uninfected mice. (B-C) Mixed bone marrow chimeric mice (1:1 mixture of WT and CD45-deficient bone marrow) were infected with 5 × 104 PFU of MCMV. (B) Histogram of representative staining for CD45 in NK1.1+CD3−Ly49H+ cells. (C) Change of percentage of CD45+ (left panel) or CD45− (right panel) cells within the Ly49H+NK1.1+CD3− splenic population in individual mice from 1 set of mixed bone marrow chimeric mice. Three independent sets of mixed bone marrow chimeras were made, with approximately 10 mice per set. Similar results were seen in all chimeric mice.
