Effect of deletion of HS-E1 on domain-wide histone modifications. A schematic of the murine β-globin locus is shown at the top, to scale but with the indicated discontinuities. The bracketed region between the ϵy-globin and βh1-globin genes shows the location of the HS-E1 deletion. Black bars beneath the locus correspond to the positions of amplimers used for ChIP analysis, and the corresponding ChIP data are shown directly below each amplimer in the bar graphs. Values in the bar graphs represent enrichments observed for the indicated histone modifications relative to inactive control loci, as measured in E12.5 primitive erythroid cells from wild-type mice (black) or from mice homozygous for the deletion of HS-E1 (gray). Note that one amplimer lies within the deleted region.