Lymphocyte CD73 instead of endothelial CD73 is important in migration of lymphocytes via afferent lymphatics. (A) Expression of CD73 on lymphocytes used for the transfer experiments. (B) Migration of subcutaneously injected wt lymphocytes (n = 10) from the skin into the draining lymph nodes of CD73-deficient and wt mice under normal conditions and after LPS challenge. (C) Migration of lymphocytes isolated from CD73-deficient (n = 5) and wt mice (n = 5) to the draining lymph nodes of CD73-deficient and wt recipients. Minimum of 5 mice were used as recipients in each combination. (D) Lymphocyte migration via the blood vessels. CFSE-labeled wt lymphocytes and TRITC-labeled CD73-deficient lymphocytes were injected into the tail vein of CD73-deficient (n = 7) and age-matched wt recipient (n = 7). Homing of the labeled lymphocytes to peripheral and mesenteric lymph nodes (LN), spleen, and blood was analyzed by flow cytometry. The results are presented as homing indexes (mean ± SEM).