CD34⁺IL-3Rα⁺ FA-AML cells give rise to leukemia in secondary recipient mice. (A) Cells (7-10 × 10⁶) isolated from the bone marrow of the primary mice transplanted with normal CD34⁺ cells, or CD34⁺ IL-3Rα⁻ or CD34⁺IL-3Rα⁺ FA-AML cells were injected intrafemorally into each NSG/SGM3 or NSG recipient mouse (n = 9 for each group). The survival of recipient mice was analyzed with a Kaplan-Meier plot. (B) Assessment of xenografts in the bone marrow of secondary recipient mice was performed by flow cytometric analysis of human CD45 staining. Each symbol represents a single mouse, and horizontal bars indicate the mean value. Note that 1 recipient mouse transplanted with normal donor cells died 6 days after transplantation, probably due to irradiation. (C-D) Representative images of spleens (C) and Giemsa-stained peripheral blood smears (D) of CD34⁺IL-3Rα⁻ or CD34⁺IL-3Rα⁺ secondary recipient mice.