Survival, chimerism, and elimination of BCL₁ tumor cells after transplantation of TCD BM with or without sorted naive or memory CD8⁺ T cells. (A) Representative 2-color flow cytometric analysis of CD19 versus BCL₁-idiotype markers in the peripheral blood from recipients 28 days after total body irradiation (800 cGy), BCL₁ cells, and a transplantation of 2 × 10⁶ TCD BM cells with or without 0.5 million naive or memory phenotype CD8⁺ T cells. Untreated control recipients were given BCL₁ only. The boxes enclose BCL₁-idiotype–positive CD19⁺ cells, and percentages of cells in boxes are shown. (B) Representative flow cytometric analysis of peripheral blood at day 28 showing percentage of donor (H-2K^b+) cells among gated TCRβ⁺ cells. (C–D) Survival of lethally irradiated BALB/c hosts given 2 × 10⁶ TCD BM cells from C57BL/6 donors with or without (n = 8) 0. 5 × 10⁶, 0.1 × 10⁶, or 0.05 × 10⁶ sorted naive (n = 15; n = 9; n = 8; C) or memory phenotype phenotype (n = 12; n = 9; n = 8; D) CD8⁺ T cells. The data were pooled from 2 independent experiments.