Amino acid sequence of the CAIX-CAR protein with identified immunoreactive epitopes. Amino acid sequence of the CAIX-CAR protein: aa 1-19, signal peptide G250 mAb VH; aa 20-137, mature VH; aa 138-159, linker; aa 160-278, mature VK; aa 279-301, CD4 transmembrane region (abbreviated CD4Tm); aa 302-347, intracellular Fc(ϵ)RI γ-chain (abbreviated γ). Identified immunoreactive epitopes are presented in bold (see also Table 3).