Treg-suppressive activity of HIV infection is mediated by contact. (A) IL-10 levels were measured in 3-day supernatants of cultured Tcons, Tregs, or Tcons + Tregs using the Luminex assay. (B) Tcons were HIV infected and cocultured with Tregs in the presence or absence of anti–IL-10R blocking antibody (10 μg/mL). Graph shows the mean (± SEM) percentage of HIV-p24^Gag+ Tcons (n = 6). (C) Graph showing the mean (± SEM) percentage of HIV-p24^Gag+ Tcons cultured alone, with Tregs, or with Tregs in a Transwell plate (n = 5).