Treg-suppressive activity of HIV infection is PKA mediated. Infected Tcons were cultured alone, in the presence of the PKA inhibitor H89 (10uM), or in the presence of a cAMP analog that specifically activates PKA (6-Bnz-cAMP, 500uM). Tcon were also cultured with Tregs alone or in the presence of 10uM H89. Tregs were loaded with calcein AM and Tcons were stained with CFSE before culture. Data represent the percentage of infected cells in gated (CFSE⁺) Tcons after 3 days. One representative experiment of 2 is shown.